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Healing & Recoverypreclinical

Thymosin Beta-4 (TB-500)

TB-500
Healing & Recovery · CAS 77591-33-4
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Category
Healing & Recovery
Evidence grade
D
PubMed records
925
Female-tagged
172
Sex-analysis
2
CAS
77591-33-4
D
Evidence grade D, Preclinical / animal evidence only across 925 indexed studies. No human trial data. How grades work →
Female evidence: None
Thymosin Beta-4 (TB-500): of 925 PubMed records, 172 are tagged as including female subjects and 2 carry a sex-analysis tag.Counts reflect PubMed records as of 2026-07-17. “Female” uses the Female MeSH filter and “sex-analysis” the Sex Factors MeSH filter, automated proxies pending manual study-level review. Methodology →

Overview

TB-500 is a synthetic peptide based on the active region of thymosin beta-4 (Tβ4), a naturally occurring actin-binding protein found widely across tissues. Its structure centers on the sequence responsible for binding monomeric actin, which gives it a focused and well-defined profile as a research material in cell-biology laboratories.

In preclinical and in-vitro research, TB-500 has been studied for its role in regulating actin polymerization, cell migration, and cytoskeletal dynamics, processes that are central to wound-healing and tissue-remodeling research models. Investigators examining how cells move and reorganize their internal scaffolding have used it as a tool compound to probe these mechanisms.

TB-500 is supplied for in-vitro and laboratory research only, not for human or veterinary use, and its evidence base is preclinical. Whether these mechanisms translate to female physiology remains an open research question; our evidence review tracks how many of the underlying studies included women.

Summary

Thymosin Beta-4 (studied as the fragment TB-500) is an actin-binding peptide investigated in cell-migration and tissue-remodeling research. Its evidence base is preclinical, and sex-stratified reporting is essentially absent.

Evidence in women

Menstrual cycle
Unstudied
No menstrual cycle interaction data.
Pregnancy & lactation
Unstudied
No pregnancy or lactation safety data exist.
Hormonal interactions
Unstudied
No hormonal interaction data.
Perimenopause & menopause
Unstudied
No data in perimenopausal or menopausal populations.
Sex differences
Unstudied
In the indexed literature, only some studies used female animals and none reported sex-stratified outcomes.

Mechanism

Binds monomeric actin and regulates actin polymerization, influencing cell migration and cytoskeletal dynamics in wound-healing research models.

Reported dosing & routes

◐

We don't give dosing or medical advice. The figures below describe what was used in published research, not a recommendation. Decide with your clinician.

Reported range (literature)Reported in animal studies only; no established human dose.
Routessubcutaneous, intramuscular
Female-specific dosingNot established

Contraindications: active malignancy (theoretical)

Side effects

Reported in the research literature. Sex-specific incidence has not been separately analysed in the available records.

insufficient human data to characterize

Thymosin Beta-4 (TB-500) reconstitution calculator

Enter this vial's amount, the bacteriostatic water you add, and a target amount to get the concentration and the U-100 syringe units to draw. Handling arithmetic for research use, not a dose recommendation.

Reconstitution

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Draw for Thymosin Beta-4 (TB-500)

5
units on a U-100 insulin syringe
020406080100
U-100 insulin syringe — 1 mL = 100 units
That's 0.05 mL drawn to the 5-unit mark.
Concentration5 mg/mL (5,000 mcg/mL)
Volume to draw0.05 mL
Doses per vial40

Reference chart at 5 mg/mL

Target amountVolume (mL)U-100 units
100 mcg0.02 mL2
250 mcg0.05 mL5
500 mcg0.1 mL10
750 mcg0.15 mL15
1000 mcg0.2 mL20

Research-use arithmetic only. This tool converts a vial amount, diluent volume, and a target amount into a concentration and syringe draw for laboratory handling. It is not dosing guidance, a recommendation, or a claim of safety or efficacy, and it does not account for overfill, dead space, or peptide purity. Nothing here is medical advice. “U-100” means 100 units = 1 mL; confirm your syringe graduation before measuring. See the reconstitution & storage handbook for handling detail.

Frequently asked questions

Answers drawn only from Thymosin Beta-4 (TB-500)'s research facts and, where reviewed, its sex-disaggregated evidence. Nothing here is medical advice, dosing, or a claim of safety or efficacy.

What is Thymosin Beta-4 (TB-500)?

Thymosin Beta-4 (TB-500), also known as TB-500, is a research compound in our healing recovery category. Thymosin Beta-4 (studied as the fragment TB-500) is an actin-binding peptide investigated in cell-migration and tissue-remodeling research.

Is Thymosin Beta-4 (TB-500) studied in women?

Thymosin Beta-4 (TB-500): of 925 PubMed records, 172 are tagged as including female subjects and 2 carry a sex-analysis tag. We grade this none: no PubMed record for Thymosin Beta-4 (TB-500) is tagged as including female subjects, so its effects in women are effectively unstudied. That blank is the finding, not a safety signal, and we do not fill it with guesses.

Has Thymosin Beta-4 (TB-500) been studied during pregnancy or breastfeeding?

Unstudied. No pregnancy or lactation safety data exist. This is not a safety clearance or a recommendation. Vera, our research chat, deliberately hard-stops on pregnancy, breastfeeding, and trying-to-conceive questions rather than answer them.

What evidence grade does Thymosin Beta-4 (TB-500) have, and why?

Thymosin Beta-4 (TB-500) carries evidence grade D. Preclinical / animal evidence only across 925 indexed studies. No human trial data.. Grades are derived from the research record, never hand-assigned; see how grades work.

Is there a female-specific dose for Thymosin Beta-4 (TB-500)?

No. A female-specific dose for Thymosin Beta-4 (TB-500) is not established, and this site does not provide dosing or medical advice. Any figures we show describe what published research used, not a recommendation. Decide anything about use with a qualified clinician.

How is Thymosin Beta-4 (TB-500) administered in research?

In the published literature, Thymosin Beta-4 (TB-500) is administered via subcutaneous, intramuscular. Reported dose ranges on the Dosing tab describe what studies used for laboratory research, not a protocol for people. We don't provide dosing.

What side effects have been reported for Thymosin Beta-4 (TB-500)?

Effects reported in the research literature include insufficient human data to characterize. Sex-specific incidence has not been separately analysed in the available records, so these are not broken out for women.

What is the CAS number for Thymosin Beta-4 (TB-500)?

The CAS registry number for Thymosin Beta-4 (TB-500) is 77591-33-4. It is also referred to as TB-500, Tβ4. Use it to confirm identity against a Certificate of Analysis.

How should Thymosin Beta-4 (TB-500) be stored and handled?

Thymosin Beta-4 (TB-500) sold for research is supplied as a lyophilized (freeze-dried) powder. Lyophilized peptides are generally kept cold and dry, at roughly -20°C for longer-term storage and 2-8°C short-term, protected from light. Once reconstituted they are typically refrigerated and used within a short window, avoiding repeated freeze-thaw cycles. This is general laboratory handling for research use only, not a directive for human use. Always follow the handling and purity details on the product's Certificate of Analysis.

Where can I buy Thymosin Beta-4 (TB-500) for research?

For laboratory research use, Thymosin Beta-4 (TB-500) is available from our research-use partner, Aurevena Research. Applying code research15 takes 15% off. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.

Buy Thymosin Beta-4 (TB-500) at Aurevena Research
Research-use supplier: Thymosin Beta-4 (TB-500) is available from our research-use vendor for laboratory use. Code research15 for 15% off, applied automatically at checkout. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.
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Educational information for laboratory and research use only. Not medical advice, a recommendation, or a claim of safety or efficacy; no personal dosing. “Unstudied” means no qualifying study was found, not that a compound is safe or unsafe.