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Hormonal & Reproductivephase_2_3

Kisspeptin-54

Metastin
Hormonal & Reproductive
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Category
Hormonal & Reproductive
Evidence grade
B
PubMed records
238
Female-tagged
127
Sex-analysis
0
CAS
—
B
Evidence grade B, Phase 2/3 human trial evidence across 238 indexed studies (mixed-sex). How grades work →
Female evidence: Direct
Kisspeptin-54: of 238 PubMed records, 127 are tagged as including female subjects and 0 carry a sex-analysis tag.Counts reflect PubMed records as of 2026-07-21. “Female” uses the Female MeSH filter and “sex-analysis” the Sex Factors MeSH filter, automated proxies pending manual study-level review. Methodology →

Overview

Kisspeptin-54 is the 54-amino-acid form of kisspeptin, a peptide that sits at the top of the reproductive hormone cascade. It has become a central research material in reproductive endocrinology because it acts upstream of the signals that drive the entire axis.

In research models, kisspeptin-54 has been studied for its association with GnRH release and the downstream secretion of luteinizing hormone and follicle-stimulating hormone. Investigators examining the reproductive axis and its regulation have used it as a primary research material for probing this upstream control point.

For a hormonal and reproductive catalog, kisspeptin-54 is a particularly relevant entry, since the reproductive axis it governs differs meaningfully between sexes. Whether specific findings extend across female physiology is exactly the kind of open question our evidence review tracks, including how many of the underlying studies included women.

Summary

Kisspeptin-54 is a reproductive-axis peptide that stimulates GnRH release, and, unusually for this database, it has been studied directly in women, including in IVF and hypothalamic amenorrhea research and in polycystic ovary syndrome. This makes it one of the few compounds here with genuine female-direct evidence.

Evidence in women

Menstrual cycle
Evidence
Kisspeptin-54 acts directly on the axis that governs the menstrual cycle and has been administered to women to probe and stimulate reproductive-hormone release.
Pregnancy & lactation
Unstudied
Kisspeptin rises in pregnancy physiologically, but kisspeptin-54 as an administered therapy is not established as safe in pregnancy or lactation.
Hormonal interactions
Evidence
It is a direct upstream regulator of LH/FSH and has been studied in women with hormonal disorders such as PCOS and hypothalamic amenorrhea.
Perimenopause & menopause
Unstudied
Reproductive-axis research has focused on women of reproductive age; perimenopausal and menopausal use is not established.
Sex differences
Evidence
Unusually for this database, kisspeptin-54 has been studied specifically in women for reproductive indications, so a female-direct evidence base exists.

Mechanism

Activates the KISS1R (GPR54) receptor on GnRH neurons to trigger gonadotropin (LH/FSH) release, the upstream driver of the reproductive axis.

Reported dosing & routes

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We don't give dosing or medical advice. The figures below describe what was used in published research, not a recommendation. Decide with your clinician.

Reported range (literature)Bolus and infusion regimens have been studied in reproductive trials (including as an IVF trigger); investigational, no approved dose.
Routessubcutaneous, intravenous
Female-specific dosingNot established

Contraindications: insufficient data to characterize outside the studied reproductive settings

Side effects

Reported in the research literature. Sex-specific incidence has not been separately analysed in the available records.

generally well tolerated in reproductive trials

Kisspeptin-54 reconstitution calculator

Enter this vial's amount, the bacteriostatic water you add, and a target amount to get the concentration and the U-100 syringe units to draw. Handling arithmetic for research use, not a dose recommendation.

Reconstitution

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Draw for Kisspeptin-54

5
units on a U-100 insulin syringe
020406080100
U-100 insulin syringe — 1 mL = 100 units
That's 0.05 mL drawn to the 5-unit mark.
Concentration5 mg/mL (5,000 mcg/mL)
Volume to draw0.05 mL
Doses per vial40

Reference chart at 5 mg/mL

Target amountVolume (mL)U-100 units
100 mcg0.02 mL2
250 mcg0.05 mL5
500 mcg0.1 mL10
750 mcg0.15 mL15
1000 mcg0.2 mL20

Research-use arithmetic only. This tool converts a vial amount, diluent volume, and a target amount into a concentration and syringe draw for laboratory handling. It is not dosing guidance, a recommendation, or a claim of safety or efficacy, and it does not account for overfill, dead space, or peptide purity. Nothing here is medical advice. “U-100” means 100 units = 1 mL; confirm your syringe graduation before measuring. See the reconstitution & storage handbook for handling detail.

Frequently asked questions

Answers drawn only from Kisspeptin-54's research facts and, where reviewed, its sex-disaggregated evidence. Nothing here is medical advice, dosing, or a claim of safety or efficacy.

What is Kisspeptin-54?

Kisspeptin-54, also known as Metastin, is a research compound in our hormonal reproductive category. Kisspeptin-54 is a reproductive-axis peptide that stimulates GnRH release, and, unusually for this database, it has been studied directly in women, including in IVF and hypothalamic amenorrhea research and in polycystic ovary syndrome.

Is Kisspeptin-54 studied in women?

Kisspeptin-54: of 238 PubMed records, 127 are tagged as including female subjects and 0 carry a sex-analysis tag. We grade this direct female evidence: at least one record carries a sex-analysis tag, so some findings are drawn from women rather than borrowed from mixed or male data. See how we read the evidence.

Has Kisspeptin-54 been studied during pregnancy or breastfeeding?

Unstudied. Kisspeptin rises in pregnancy physiologically, but kisspeptin-54 as an administered therapy is not established as safe in pregnancy or lactation. This is not a safety clearance or a recommendation. Vera, our research chat, deliberately hard-stops on pregnancy, breastfeeding, and trying-to-conceive questions rather than answer them.

What evidence grade does Kisspeptin-54 have, and why?

Kisspeptin-54 carries evidence grade B. Phase 2/3 human trial evidence across 238 indexed studies (mixed-sex).. Grades are derived from the research record, never hand-assigned; see how grades work.

Is there a female-specific dose for Kisspeptin-54?

No. A female-specific dose for Kisspeptin-54 is not established, and this site does not provide dosing or medical advice. Any figures we show describe what published research used, not a recommendation. Decide anything about use with a qualified clinician.

How is Kisspeptin-54 administered in research?

In the published literature, Kisspeptin-54 is administered via subcutaneous, intravenous. Reported dose ranges on the Dosing tab describe what studies used for laboratory research, not a protocol for people. We don't provide dosing.

What side effects have been reported for Kisspeptin-54?

Effects reported in the research literature include generally well tolerated in reproductive trials. Sex-specific incidence has not been separately analysed in the available records, so these are not broken out for women.

How should Kisspeptin-54 be stored and handled?

Kisspeptin-54 sold for research is supplied as a lyophilized (freeze-dried) powder. Lyophilized peptides are generally kept cold and dry, at roughly -20°C for longer-term storage and 2-8°C short-term, protected from light. Once reconstituted they are typically refrigerated and used within a short window, avoiding repeated freeze-thaw cycles. This is general laboratory handling for research use only, not a directive for human use. Always follow the handling and purity details on the product's Certificate of Analysis.

Where can I buy Kisspeptin-54 for research?

Kisspeptin-54 is not currently listed by our research-use partner, Aurevena Research, but their catalog carries related compounds. Code research15 applies 15% off. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.

Browse the Aurevena catalog
Research-use supplier: Kisspeptin-54 isn't currently listed by our research-use vendor. Their catalog carries related compounds. Code research15 for 15% off, applied automatically at checkout. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.
Browse the catalog →

Citations (PMID)

31515170 · 15519892

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Educational information for laboratory and research use only. Not medical advice, a recommendation, or a claim of safety or efficacy; no personal dosing. “Unstudied” means no qualifying study was found, not that a compound is safe or unsafe.