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Longevitypreclinical

PNC-27

p53-HDM2 membrane peptide
Longevity
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Category
Longevity
Evidence grade
D
PubMed records
23
Female-tagged
7
Sex-analysis
0
CAS
—
D
Evidence grade D, Preclinical / animal evidence only across 23 indexed studies. No human trial data. How grades work →
Female evidence: None
PNC-27: of 23 PubMed records, 7 are tagged as including female subjects and 0 carry a sex-analysis tag.Counts reflect PubMed records as of 2026-07-21. “Female” uses the Female MeSH filter and “sex-analysis” the Sex Factors MeSH filter, automated proxies pending manual study-level review. Methodology →

Overview

PNC-27 is a synthetic 32-amino-acid peptide that fuses the p53 HDM-2-binding domain to a penetratin-like membrane-residency sequence, and it is used as a tool compound in cancer-cell research. This design combines a defined protein-interaction motif with a membrane-targeting element in a single research material.

In vitro and in preclinical systems, PNC-27 has been studied for its modeled selective interaction with HDM-2 expressed in the membranes of transformed cells, where investigators examine membrane-pore formation and necrosis- and apoptosis-related mechanisms. Researchers working on p53-HDM-2 biology have used it to probe how this interaction behaves at the cell membrane rather than the nucleus.

Supplied for in-vitro and laboratory research only, PNC-27 is a useful catalog entry because it isolates a specific, membrane-localized mechanism of interest in oncology research. Whether these mechanisms translate to female physiology remains an open research question; our evidence review tracks how many of the underlying studies included women.

Summary

PNC-27 is a synthetic peptide combining a p53 HDM-2-binding domain with a membrane-penetrating sequence, studied for selectively killing cancer cells. All evidence is preclinical; there are no human trials and no female-specific data.

Evidence in women

Menstrual cycle
Unstudied
No studies examine menstrual cycle interactions.
Pregnancy & lactation
Unstudied
No pregnancy or lactation safety data exist.
Hormonal interactions
Unstudied
No data on reproductive- or thyroid-hormone interactions.
Perimenopause & menopause
Unstudied
No perimenopausal or menopausal data.
Sex differences
Unstudied
Preclinical cancer-model studies did not report sex-stratified outcomes.

Mechanism

Binds HDM-2 in the cancer-cell membrane and forms transmembrane pores, causing necrosis reported to spare normal cells in preclinical work.

Reported dosing & routes

◐

We don't give dosing or medical advice. The figures below describe what was used in published research, not a recommendation. Decide with your clinician.

Reported range (literature)No established human dose; anticancer activity shown in preclinical models.
Routesintravenous, intraperitoneal
Female-specific dosingNot established

Contraindications: insufficient data to characterize

Side effects

Reported in the research literature. Sex-specific incidence has not been separately analysed in the available records.

insufficient human data to characterize

PNC-27 reconstitution calculator

Enter this vial's amount, the bacteriostatic water you add, and a target amount to get the concentration and the U-100 syringe units to draw. Handling arithmetic for research use, not a dose recommendation.

Reconstitution

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Draw for PNC-27

5
units on a U-100 insulin syringe
020406080100
U-100 insulin syringe — 1 mL = 100 units
That's 0.05 mL drawn to the 5-unit mark.
Concentration5 mg/mL (5,000 mcg/mL)
Volume to draw0.05 mL
Doses per vial40

Reference chart at 5 mg/mL

Target amountVolume (mL)U-100 units
100 mcg0.02 mL2
250 mcg0.05 mL5
500 mcg0.1 mL10
750 mcg0.15 mL15
1000 mcg0.2 mL20

Research-use arithmetic only. This tool converts a vial amount, diluent volume, and a target amount into a concentration and syringe draw for laboratory handling. It is not dosing guidance, a recommendation, or a claim of safety or efficacy, and it does not account for overfill, dead space, or peptide purity. Nothing here is medical advice. “U-100” means 100 units = 1 mL; confirm your syringe graduation before measuring. See the reconstitution & storage handbook for handling detail.

Frequently asked questions

Answers drawn only from PNC-27's research facts and, where reviewed, its sex-disaggregated evidence. Nothing here is medical advice, dosing, or a claim of safety or efficacy.

What is PNC-27?

PNC-27, also known as p53-HDM2 membrane peptide, is a research compound in our longevity category. PNC-27 is a synthetic peptide combining a p53 HDM-2-binding domain with a membrane-penetrating sequence, studied for selectively killing cancer cells.

Is PNC-27 studied in women?

PNC-27: of 23 PubMed records, 7 are tagged as including female subjects and 0 carry a sex-analysis tag. We grade this none: no PubMed record for PNC-27 is tagged as including female subjects, so its effects in women are effectively unstudied. That blank is the finding, not a safety signal, and we do not fill it with guesses.

Has PNC-27 been studied during pregnancy or breastfeeding?

Unstudied. No pregnancy or lactation safety data exist. This is not a safety clearance or a recommendation. Vera, our research chat, deliberately hard-stops on pregnancy, breastfeeding, and trying-to-conceive questions rather than answer them.

What evidence grade does PNC-27 have, and why?

PNC-27 carries evidence grade D. Preclinical / animal evidence only across 23 indexed studies. No human trial data.. Grades are derived from the research record, never hand-assigned; see how grades work.

Is there a female-specific dose for PNC-27?

No. A female-specific dose for PNC-27 is not established, and this site does not provide dosing or medical advice. Any figures we show describe what published research used, not a recommendation. Decide anything about use with a qualified clinician.

How is PNC-27 administered in research?

In the published literature, PNC-27 is administered via intravenous, intraperitoneal. Reported dose ranges on the Dosing tab describe what studies used for laboratory research, not a protocol for people. We don't provide dosing.

What side effects have been reported for PNC-27?

Effects reported in the research literature include insufficient human data to characterize. Sex-specific incidence has not been separately analysed in the available records, so these are not broken out for women.

How should PNC-27 be stored and handled?

PNC-27 sold for research is supplied as a lyophilized (freeze-dried) powder. Lyophilized peptides are generally kept cold and dry, at roughly -20°C for longer-term storage and 2-8°C short-term, protected from light. Once reconstituted they are typically refrigerated and used within a short window, avoiding repeated freeze-thaw cycles. This is general laboratory handling for research use only, not a directive for human use. Always follow the handling and purity details on the product's Certificate of Analysis.

Where can I buy PNC-27 for research?

For laboratory research use, PNC-27 is available from our research-use partner, Aurevena Research. Applying code research15 takes 15% off. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.

Buy PNC-27 at Aurevena Research
Research-use supplier: PNC-27 is available from our research-use vendor for laboratory use. Code research15 for 15% off, applied automatically at checkout. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.
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Citations (PMID)

21728981 · 38927351

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Educational information for laboratory and research use only. Not medical advice, a recommendation, or a claim of safety or efficacy; no personal dosing. “Unstudied” means no qualifying study was found, not that a compound is safe or unsafe.